A Cards_Caspase-1 version was engineered to avoid self-processing but cleavage by thrombin allow, where residues 99C103 (including self-processing sites E102/D103) were mutated to a thrombin cleavage site (LVPRG), and residues E121 and D122 were substituted with alanine to avoid self-processing
A Cards_Caspase-1 version was engineered to avoid self-processing but cleavage by thrombin allow, where residues 99C103 (including self-processing sites E102/D103)…