Links to PubMed will also be available for Selected Referrals. ? 202 203 204 205 206 207 208 209 210 211 212 ? Images in this article Image on p.204 Image on p.205 Image on p.206 Image on p.206 Image on p.207 Image on p.208 Click on the image to see a larger version. Selected. or intrinsically with [35S]methionine. Analysis of two-dimensional gels by immunoblotting with rabbit antisera prepared from protein I bands removed from a polyacrylamide gel exposed the presence of Vardenafil antigens in the same area of the gel (below proteins that were 23,000 to 36,000 in Mr). Antibodies to constituents Ptprc which migrated below the diagonal were essentially eliminated by adsorption of antisera with purified LPS, as were antibodies to homologous LPS and LPS in proteinase K-digested whole-cell lysates. Immunoblotting having a monoclonal antibody specific for LPS shown reactivity of the antibody with LPS and with the protein I band. On the basis of these data, we conclude that protein I and perhaps additional proteins in the whole-cell lysate are stably associated with LPS; this complex is definitely resistant to dissociation in sodium dodecyl sulfate at high temperature (approximately 100 degrees Vardenafil C) but does, for unknown reasons, dissociate with electrophoresis in the second dimensions. The association of LPS with protein antigens in sodium dodecyl sulfate-polyacrylamide gels adds another dimensions of difficulty to analysis of these antigens by immunoelectroblotting. Furthermore, the limited association of LPS with the major outer membrane protein I may alter the nature of the immune response generated by “purified” protein I vaccine antigens. The possible part of protein-LPS complexes in the pathogenesis of gonorrhea is definitely discussed. Vardenafil Full text Full text is definitely available like a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (3.3M), or click on a page image below to browse page by page. Links to PubMed will also be available for Selected Referrals. ? 202 203 204 205 206 207 208 209 210 211 212 ? Images in this article Image on p.204 Image on p.205 Image on p.206 Image on p.206 Image on p.207 Image on p.208 Click on the image to see a larger version. Selected.